MST4 polyclonal antibody
产品名称： MST4 polyclonal antibody
英文名称： MST4 polyclonal antibody
产品编号： PAB2109
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of MST4.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MST4.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- The Ste20 kinase MST4 plays a role in prostate cancer progression.
Sung V, Luo W, Qian D, Lee I, Jallal B, Gishizky M.Cancer Res. 2003 Jun 15;63(12):3356-63.
- 2.
- Phosphorylation and dimerization regulate nucleocytoplasmic shuttling of mammalian STE20-like kinase (MST).
Lee KK, Yonehara S.J Biol Chem. 2002 Apr 5;277(14):12351-8. Epub 2002 Jan 22.
- 3.
- Cloning of MASK, a novel member of the mammalian germinal center kinase III subfamily, with apoptosis-inducing properties.
Dan I, Ong SE, Watanabe NM, Blagoev B, Nielsen MM, Kajikawa E, Kristiansen TZ, Mann M, Pandey A.J Biol Chem. 2002 Feb 22;277(8):5929-39. Epub 2001 Dec 6.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of MST4 polyclonal antibody (Cat # PAB2109) in A-549 cell lysate. MST4 (arrow) was detected using purified Polyclonal antibody.
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MST4 polyclonal antibody (Cat # PAB2109) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 51765
- Protein Accession#:
- NP_057626
- Gene Name:
- RP6-213H19.1
- Gene Alias:
- MASK,MST4
- Gene Description:
- serine/threonine protein kinase MST4
- Omim ID:
- 300547
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq
- Other Designations:
- Mst3 and SOK1-related kinase,STE20-like kinase MST4,mammalian Ste20-like protein kinase 4,mammalian sterile 20-like 4,serine/threonine protein kinase MASK