RHEB polyclonal antibody
产品名称： RHEB polyclonal antibody
英文名称： RHEB polyclonal antibody
产品编号： PAB4475
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of RHEB.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to internal region of human RHEB.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Phospholipase D1 is an effector of Rheb in the mTOR pathway.
Sun Y, Fang Y, Yoon MS, Zhang C, Roccio M, Zwartkruis FJ, Armstrong M, Brown HA, Chen J.Proc Natl Acad Sci U S A. 2008 Jun 17;105(24):8286-91. Epub 2008 Jun 11.
- 2.
- Rheb activates mTOR by antagonizing its endogenous inhibitor, FKBP38.
Bai X, Ma D, Liu A, Shen X, Wang QJ, Liu Y, Jiang Y.Science. 2007 Nov 9;318(5852):977-80.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of RHEB polyclonal antibody (Cat # PAB4475) in CEM cell line lysates (35 ug/lane). RHEB (arrow) was detected using the purified RHEB polyclonal antibody (Cat # PAB4475) (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human lung carcinomareacted with RHEB polyclonal antibody (Cat # PAB4475), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 6009
- Protein Accession#:
- NP_005605;Q15382
- Gene Name:
- RHEB
- Gene Alias:
- MGC111559,RHEB2
- Gene Description:
- Ras homolog enriched in brain
- Omim ID:
- 601293
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq
- Other Designations:
- GTP-binding protein Rheb,Ras homolog enriched in brain 2
- Gene Pathway
- Related Disease